Preparative SFC: Mixed vs. Modifier-Stream Injections

This presentation, from the Thar (Waters) SFC Users Group meeting in Zurich in 2008, was uploaded in response to questions about the effect of injecting samples on columns in liquid solvents.  In SFC (unlike HPLC), the mobile phase is primarily liquid CO₂, and injecting samples dissolved in mobile phase is impossible. The effect of increasing injection volume in particular is dramatic.

Miller & Sebastian, at Amgen, later published aspects of our work in a 2012 paper in J. Chromatogr. A.  More recently, scientists at Waters Corporation have extended the study of diluent effects to varying the solvent chemically.