Using Chiral SFC/MS to Solve a Bioanalysis Problem
November 29, 2012
We were asked recently to participate in a technology forum on “Analytical Applications” for Pharmaceutical Technology magazine’s online edition (PharmTech.com).
In this technical forum industry experts, including Sharanya Reddy (PerkinElmer), Robert Mattes (Foss NIRSystems), and Stephen Hoag & Ravikanth Kona (University of Maryland, Dept. Pharmaceutical Sciences), offer case studies in pharmaceutical analysis.
Our team presented an example in which chiral SFC–mass spectrometry (MS) is shown to be an expedient analytical approach to solving a bioanalysis problem. SFC–MS, used with a chiral separation method identical to the one used to produce the tested enantiomers, proved useful for studying their potential racemization in pharmacokinetic studies. In addition to chiral SFC-MS, case studies were presented using near-infrared spectroscopy (NIRS) and time-of-flight (TOF) mass spectrometry (MS).
This is an interesting area right now, made more so by recently available analytical SFC systems from chromatography instrument vendors. Fun times for chiral drug discovery work!