EPSA Assay 101: Background, Best Practices, & Success Stories
EPSA is a supercritical fluid chromatography (SFC) technique for peptides that enables improved permeability design and can be used as a permeability surrogate. This method is used to assess polarity by retention time using controlled SFC conditions in a standardized method.
In this webinar, Gilles Goetz, Ph.D., an original developer of EPSA, presents the background and preliminary implementation. We then hear from professionals in the industry about their own experiences using EPSA through a panel discussion and Q+A session.
To watch the webinar, simply fill out the form on the right.
About the Presenters
Gilles Goetz, Ph.D., Principal Scientist, Pfizer
Co-developer of the EPSA Assay, Gilles presents the background of EPSA and weighs in on how it is being implemented today.
Bill Farrell, Associate Research Fellow, Pfizer
Co-developer of the EPSA Assay, Bill discusses the assay’s new niche for large molecules and peptides alongside its original use for small molecules. Bill is the moderator of this event and weighs in during the Q+A session.
Romulo Romero, Analytical Scientist, AbbVie
Romulo is an analytical chemist with over 20 years in pharma discovery, currently focusing on SFC, with an interest in automation and high throughput processes, as well as space exploration.
Huy Nguyen, Scientist, Genentech
Huy is a scientist in the Analytical Research group from Genentech. His group focuses on running phys chem assays like EPSA, logD, and solubility. In addition, Huy’s group utilizes microfluidics to develop novel phys chem assays.
Johan Nicolaï, Sr. Research Scientist Investigative ADME, UCB
Johan is a senior scientist in the DMPK department at UCB. After obtaining his PhD, he joined UCB in 2016 and focused on the prediction of brain disposition of small molecules before moving to the area of intestinal drug absorption of NCEs. More recently he has been involved in the evaluation of the permeability of bRo5 compounds and is looking at ways to relate bRo5 compounds’ passive permeability to physicochemical parameters such as EPSA.
Erin Jordan, Sr. Scientist I, AbbVie
Erin received her Bachelors degree from the University of MN and her Masters in Chemistry from Northwestern University, performing synthetic organic chemistry under the advisory of Karl Scheidt. Erin began her career at Abbott Laboratories in 2009 providing chiral purification support to Process Chemistry. In 2011 she transitioned into Abbott/AbbVie Drug Discovery where she currently co-leads the chiral SFC platform supporting medicinal chemistry therapeutic teams. Erin has been running the EPSA assay within her group since 2018.
Paul Lefebvre, General Manager, Averica Discovery
General Manager of Averica Discovery since 2018, Paul has worked in separations and purification for over 25 years. His experience with purification has been as both a vendor and a customer. Joining Averica in 2008, he has worked to grow the company into a full service chromatography CRO including purification by SFC and HPLC.